ap-2 gamma antibody Search Results


91
Bioss bs 6694r
Antibodies used in this experiment.
Bs 6694r, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation ap-2 gamma antibody
Antibodies used in this experiment.
Ap 2 Gamma Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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94
R&D Systems ap2 gamma
Antibodies used in this experiment.
Ap2 Gamma, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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93
Proteintech anti tfap2c
Antibodies used in this experiment.
Anti Tfap2c, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Novus Biologicals anti ap 2
Antibodies used in this experiment.
Anti Ap 2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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94
R&D Systems goat monoclonal tfap2c
a, immunofluorescence images validating correct expression of TSC marker genes in colonies of a WIBR3 (W3) naïve hESC derived TSC line (termed nTSC). CK7, <t>TFAP2C,</t> GATA3, CDX2 (all in green); DAPI (blue). Scale bar, 100 µm. b, scheme for aggregation protocol of naïve pluripotent stem cells (nPSCs) in HENSM media, naïve-derived trophoblast stem cells (nTSCs), and nPSCs induced in RCL towards PrE/ExEM for 3 days. c , immunofluorescence images showing rare expression of CK7, GATA3, and SDC1 (red) trophoblast markers in the aggregates. nuclei (DAPI, white); Left column scale bar, 500 µm; Right, zoom into several aggregates with CK7 expression; scale bar, 100 µm. d , immunofluorescence image from (upper left panel in c) , showing Epi (OCT4, cyan) and PrE (SOX17, yellow) with CK7 (red); nuclei (DAPI, white). Zoom ins from the indicated regions are shown. Although some aggregates express lineage markers, they do not organize into embryoid-like structures and are not uniformly surrounded by the trophoblast compartment. Scale bar, 500 µm; bottom, 100 µm.
Goat Monoclonal Tfap2c, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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85
Proteintech anti g2 cop antibody
a, immunofluorescence images validating correct expression of TSC marker genes in colonies of a WIBR3 (W3) naïve hESC derived TSC line (termed nTSC). CK7, <t>TFAP2C,</t> GATA3, CDX2 (all in green); DAPI (blue). Scale bar, 100 µm. b, scheme for aggregation protocol of naïve pluripotent stem cells (nPSCs) in HENSM media, naïve-derived trophoblast stem cells (nTSCs), and nPSCs induced in RCL towards PrE/ExEM for 3 days. c , immunofluorescence images showing rare expression of CK7, GATA3, and SDC1 (red) trophoblast markers in the aggregates. nuclei (DAPI, white); Left column scale bar, 500 µm; Right, zoom into several aggregates with CK7 expression; scale bar, 100 µm. d , immunofluorescence image from (upper left panel in c) , showing Epi (OCT4, cyan) and PrE (SOX17, yellow) with CK7 (red); nuclei (DAPI, white). Zoom ins from the indicated regions are shown. Although some aggregates express lineage markers, they do not organize into embryoid-like structures and are not uniformly surrounded by the trophoblast compartment. Scale bar, 500 µm; bottom, 100 µm.
Anti G2 Cop Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
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N/A
The AP-2 gamma Antibody [Alexa Fluor® 700] from Novus is a AP-2 gamma antibody to AP-2 gamma. This antibody reacts with Human. The AP-2 gamma antibody has been validated for the following applications: Western Blot.
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N/A
The AP-2 gamma Antibody [DyLight 594] from Novus is a AP-2 gamma antibody to AP-2 gamma. This antibody reacts with Human. The AP-2 gamma antibody has been validated for the following applications: Western Blot.
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N/A
The AP-2 gamma Antibody [DyLight 650] from Novus is a AP-2 gamma antibody to AP-2 gamma. This antibody reacts with Human. The AP-2 gamma antibody has been validated for the following applications: Western Blot.
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N/A
The AP-2 gamma Antibody [FITC] from Novus is a AP-2 gamma antibody to AP-2 gamma. This antibody reacts with Human. The AP-2 gamma antibody has been validated for the following applications: Western Blot.
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Image Search Results


Antibodies used in this experiment.

Journal: Frontiers in Cell and Developmental Biology

Article Title: ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2

doi: 10.3389/fcell.2021.712224

Figure Lengend Snippet: Antibodies used in this experiment.

Article Snippet: CDX2 , Bioss , bs-6694R.

Techniques:

a, immunofluorescence images validating correct expression of TSC marker genes in colonies of a WIBR3 (W3) naïve hESC derived TSC line (termed nTSC). CK7, TFAP2C, GATA3, CDX2 (all in green); DAPI (blue). Scale bar, 100 µm. b, scheme for aggregation protocol of naïve pluripotent stem cells (nPSCs) in HENSM media, naïve-derived trophoblast stem cells (nTSCs), and nPSCs induced in RCL towards PrE/ExEM for 3 days. c , immunofluorescence images showing rare expression of CK7, GATA3, and SDC1 (red) trophoblast markers in the aggregates. nuclei (DAPI, white); Left column scale bar, 500 µm; Right, zoom into several aggregates with CK7 expression; scale bar, 100 µm. d , immunofluorescence image from (upper left panel in c) , showing Epi (OCT4, cyan) and PrE (SOX17, yellow) with CK7 (red); nuclei (DAPI, white). Zoom ins from the indicated regions are shown. Although some aggregates express lineage markers, they do not organize into embryoid-like structures and are not uniformly surrounded by the trophoblast compartment. Scale bar, 500 µm; bottom, 100 µm.

Journal: bioRxiv

Article Title: Transgene-Free Ex Utero Derivation of A Human Post-Implantation Embryo Model Solely from Genetically Unmodified Naïve PSCs

doi: 10.1101/2023.06.14.544922

Figure Lengend Snippet: a, immunofluorescence images validating correct expression of TSC marker genes in colonies of a WIBR3 (W3) naïve hESC derived TSC line (termed nTSC). CK7, TFAP2C, GATA3, CDX2 (all in green); DAPI (blue). Scale bar, 100 µm. b, scheme for aggregation protocol of naïve pluripotent stem cells (nPSCs) in HENSM media, naïve-derived trophoblast stem cells (nTSCs), and nPSCs induced in RCL towards PrE/ExEM for 3 days. c , immunofluorescence images showing rare expression of CK7, GATA3, and SDC1 (red) trophoblast markers in the aggregates. nuclei (DAPI, white); Left column scale bar, 500 µm; Right, zoom into several aggregates with CK7 expression; scale bar, 100 µm. d , immunofluorescence image from (upper left panel in c) , showing Epi (OCT4, cyan) and PrE (SOX17, yellow) with CK7 (red); nuclei (DAPI, white). Zoom ins from the indicated regions are shown. Although some aggregates express lineage markers, they do not organize into embryoid-like structures and are not uniformly surrounded by the trophoblast compartment. Scale bar, 500 µm; bottom, 100 µm.

Article Snippet: The antibodies and dilutions employed for cell immunofluorescence were the following: Rabbit polyclonal anti-Cdx2 (Cell Signaling Cat# 3977) 1:200; Mouse monoclonal anti-Cdx2 (Biogenex Cat# MU392A-UC) 1:200; Rabbit polyclonal anti Gata4 (Abcam Cat# Ab84593) 1:120; Rabbit monoclonal anti-Foxa2 (Abcam Cat# Ab108422) 1:100; Goat polyclonal anti-Sox17 (R&D Cat# AF1924) 1:200; Mouse monoclonal anti-Oct4 (clone C-10) (Santa Cruz Cat# SC-5279) 1:200; Rabbit monoclonal Cdx2 (Abcam Cat# ab76541) 1:200; Goat Monoclonal Tfap2c (R&D Cat# AF5059) 1:200; Rabbit monoclonal Cytokeratin 7 (Abcam Cat# ab181598) 1:200; Rabbit monoclonal Cytokeratin 7 (Abcam Cat# ab68459) 1:200; Rabbit monoclonal Nanog (Abcam Cat# ab109250) 1:200; Mouse monoclonal Gata3 (Invitrogen Cat# MA1-028) 1:200; Goat polyclonal Gata3 (R&D Cat# AF2605) 1:200; Mouse monoclonal HCG-Beta (Abcam Cat# ab9582) 1:200; Rabbit monoclonal Gata6 (Cell signaling Cat# 5951) 1:200; Goat polyclonal Oct3/4 (R&D Cat# AF1759) 1:200; Goat polyclonal Gata6 (R&D Cat# AF1700) 1:200; Rabbit monoclonal Syndecan1 (Abcam Cat# ab128936) 1:500; Rabbit monoclonal PDGFR-A (Abcam Cat# ab134123) 1:100; Goat polyclonal Nidogen2 (R&D Cat# AF3385) 1:100.

Techniques: Immunofluorescence, Expressing, Marker, Derivative Assay

a, scheme of the donor plasmid vector used for genomic integration of the DOX-inducible iGATA3 overexpression transgene. b , immunofluorescence images of iGATA3 cells, showing uniform GATA3 expression (green) only in response to DOX; nuclei (DAPI, blue). Scale bars, 100 µm. c , brightfield images of WT (top) and iGATA3 (bottom) cells after incubation in BAP(J) media for three days. Scale bar, 200 µm. d , qRT-PCR gene expression (normalized by GAPDH and ACTIN) of the trophoblast markers for WT naïve pluripotent stem cells (nPSCs) in BAP(J) media (purple) and iGATA3 nPSC cells induced by DOX in BAP(J) media (yellow), versus nPSCs maintained in HENSM media and used as a reference control (set as 1) (white). e , immunofluorescence images showing different patterns of expression of trophoblast marker genes in the wild type (WT) nPSCs incubated in BAP(J) media for three days (top) versus iGATA3 cells, induced by DOX in BAP(J) media (bottom). GATA3 (magenta), TFAP2C (magenta), GATA2, CDX2, SDC1, and HCGB (all in green), nuclei (DAPI, blue). Scale bar, 100 µm. f , immunofluorescence images showing uniform expression of CK7 in colonies of WT nPSCs incubated in BAP(J) media for three days (top), whereas colonies of iGATA3 cells induced by DOX in BAP(J) media express CK7 heterogeneously, with most of them being negative for CK7. Scale bar, 100 µm. g , from left to right, FACS plots of ENPEP versus TACSTD2 for trophoblast (Tb) priming using iGATA3 induction in different media (AP(J) with and without DOX, BAP(J) with and without DOX), and using WT nPSC priming to trophectoderm in AP(J) and BAP(J) regimens. Percentage of double positive population is indicated. This result shows that transient expression of the GATA3 transgene is not required for Tb differentiation and that it can be achieved with BAP(J) media using WT nPSCs as starting cells.

Journal: bioRxiv

Article Title: Transgene-Free Ex Utero Derivation of A Human Post-Implantation Embryo Model Solely from Genetically Unmodified Naïve PSCs

doi: 10.1101/2023.06.14.544922

Figure Lengend Snippet: a, scheme of the donor plasmid vector used for genomic integration of the DOX-inducible iGATA3 overexpression transgene. b , immunofluorescence images of iGATA3 cells, showing uniform GATA3 expression (green) only in response to DOX; nuclei (DAPI, blue). Scale bars, 100 µm. c , brightfield images of WT (top) and iGATA3 (bottom) cells after incubation in BAP(J) media for three days. Scale bar, 200 µm. d , qRT-PCR gene expression (normalized by GAPDH and ACTIN) of the trophoblast markers for WT naïve pluripotent stem cells (nPSCs) in BAP(J) media (purple) and iGATA3 nPSC cells induced by DOX in BAP(J) media (yellow), versus nPSCs maintained in HENSM media and used as a reference control (set as 1) (white). e , immunofluorescence images showing different patterns of expression of trophoblast marker genes in the wild type (WT) nPSCs incubated in BAP(J) media for three days (top) versus iGATA3 cells, induced by DOX in BAP(J) media (bottom). GATA3 (magenta), TFAP2C (magenta), GATA2, CDX2, SDC1, and HCGB (all in green), nuclei (DAPI, blue). Scale bar, 100 µm. f , immunofluorescence images showing uniform expression of CK7 in colonies of WT nPSCs incubated in BAP(J) media for three days (top), whereas colonies of iGATA3 cells induced by DOX in BAP(J) media express CK7 heterogeneously, with most of them being negative for CK7. Scale bar, 100 µm. g , from left to right, FACS plots of ENPEP versus TACSTD2 for trophoblast (Tb) priming using iGATA3 induction in different media (AP(J) with and without DOX, BAP(J) with and without DOX), and using WT nPSC priming to trophectoderm in AP(J) and BAP(J) regimens. Percentage of double positive population is indicated. This result shows that transient expression of the GATA3 transgene is not required for Tb differentiation and that it can be achieved with BAP(J) media using WT nPSCs as starting cells.

Article Snippet: The antibodies and dilutions employed for cell immunofluorescence were the following: Rabbit polyclonal anti-Cdx2 (Cell Signaling Cat# 3977) 1:200; Mouse monoclonal anti-Cdx2 (Biogenex Cat# MU392A-UC) 1:200; Rabbit polyclonal anti Gata4 (Abcam Cat# Ab84593) 1:120; Rabbit monoclonal anti-Foxa2 (Abcam Cat# Ab108422) 1:100; Goat polyclonal anti-Sox17 (R&D Cat# AF1924) 1:200; Mouse monoclonal anti-Oct4 (clone C-10) (Santa Cruz Cat# SC-5279) 1:200; Rabbit monoclonal Cdx2 (Abcam Cat# ab76541) 1:200; Goat Monoclonal Tfap2c (R&D Cat# AF5059) 1:200; Rabbit monoclonal Cytokeratin 7 (Abcam Cat# ab181598) 1:200; Rabbit monoclonal Cytokeratin 7 (Abcam Cat# ab68459) 1:200; Rabbit monoclonal Nanog (Abcam Cat# ab109250) 1:200; Mouse monoclonal Gata3 (Invitrogen Cat# MA1-028) 1:200; Goat polyclonal Gata3 (R&D Cat# AF2605) 1:200; Mouse monoclonal HCG-Beta (Abcam Cat# ab9582) 1:200; Rabbit monoclonal Gata6 (Cell signaling Cat# 5951) 1:200; Goat polyclonal Oct3/4 (R&D Cat# AF1759) 1:200; Goat polyclonal Gata6 (R&D Cat# AF1700) 1:200; Rabbit monoclonal Syndecan1 (Abcam Cat# ab128936) 1:500; Rabbit monoclonal PDGFR-A (Abcam Cat# ab134123) 1:100; Goat polyclonal Nidogen2 (R&D Cat# AF3385) 1:100.

Techniques: Plasmid Preparation, Over Expression, Immunofluorescence, Expressing, Incubation, Quantitative RT-PCR, Marker